Overview

  • Product nameAnti-IKB beta antibody
    See all IKB beta primary antibodies
  • Description
    Mouse polyclonal to IKB beta
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fusion protein (tagged): EKLYAAGAGV LVTERGGHTA LHLACRVRAH TCAYVLLQPR PSHPRDASDT YLTQSQDHTP DTSHAPVATD PQPNPGNEEE LRDEDWRLQL EAENYDGHTP , corresponding to amino acids 111-210 of Rat IKK beta

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab52775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • Tissue specificityExpressed in all tissues examined.
  • Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications
    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B beta antibody
    • I-kappa-B-beta antibody
    • IkappaBbeta antibody
    • IKB beta antibody
    • IkB-B antibody
    • IkB-beta antibody
    • IKBB antibody
    • IKBB_HUMAN antibody
    • IkBbeta antibody
    • NF kappa BIB antibody
    • NF-kappa-B inhibitor beta antibody
    • NF-kappa-BIB antibody
    • Nfkbib antibody
    • Thyroid receptor interacting protein 9 antibody
    • Thyroid receptor-interacting protein 9 antibody
    • TR interacting protein 9 antibody
    • TR-interacting protein 9 antibody
    • TRIP-9 antibody
    • TRIP9 antibody
    see all

Anti-IKB beta antibody images

  • All lanes : Anti-IKB beta antibody (ab52775) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen.
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-IKB beta antibody (ab52775)

ab52775 has not yet been referenced specifically in any publications.

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