Overview

  • Product name
    Anti-IKB beta antibody [EPR5037]
    See all IKB beta primary antibodies
  • Description
    Rabbit monoclonal [EPR5037] to IKB beta
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human IKB beta.

  • Positive control
    • Jurkat, HeLa, THP-1, and MCF7 cell lysates, Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
    • Tissue specificity
      Expressed in all tissues examined.
    • Sequence similarities
      Belongs to the NF-kappa-B inhibitor family.
      Contains 6 ANK repeats.
    • Post-translational
      modifications
      Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • I kappa B beta antibody
      • I-kappa-B-beta antibody
      • IkappaBbeta antibody
      • IKB beta antibody
      • IkB-B antibody
      • IkB-beta antibody
      • IKBB antibody
      • IKBB_HUMAN antibody
      • IkBbeta antibody
      • NF kappa BIB antibody
      • NF-kappa-B inhibitor beta antibody
      • NF-kappa-BIB antibody
      • Nfkbib antibody
      • Thyroid receptor interacting protein 9 antibody
      • Thyroid receptor-interacting protein 9 antibody
      • TR interacting protein 9 antibody
      • TR-interacting protein 9 antibody
      • TRIP-9 antibody
      • TRIP9 antibody
      see all

    Anti-IKB beta antibody [EPR5037] images



    • Predicted band size : 37 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: IKB beta knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab109509 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab109509 was shown to specifically react with IKB beta in wild type cells as signal was lost in IKB beta knockout cells. Wild-type and IKB beta knockout samples were subjected to SDS-PAGE. ab109509 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109509 at a dilution of 1/100.
    • All lanes : Anti-IKB beta antibody [EPR5037] (ab109509) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References for Anti-IKB beta antibody [EPR5037] (ab109509)

    This product has been referenced in:
    • Yi L  et al. Inflammation-mediated SOD-2 upregulation contributes to epithelial-mesenchymal transition and migration of tumor cells in aflatoxin G1-induced lung adenocarcinoma. Sci Rep 7:7953 (2017). Read more (PubMed: 28801561) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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