The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family. Contains 6 ANK repeats.
Post-translational modificationsPhosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
ab75746, at a 1/50 dilution, staining IkB beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
References for Anti-IkB beta (phospho S23) antibody (ab75746)
This product has been referenced in:
Kane J et al. Hematopoietic stem and progenitor cell migration after hypofractionated radiation therapy in a murine model. Int J Radiat Oncol Biol Phys87:1162-70 (2013).
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