Anti-IkB beta (phospho T19) antibody (ab63510)

Overview

  • Product nameAnti-IkB beta (phospho T19) antibody
    See all IKB beta primary antibodies
  • Description
    Rabbit polyclonal to IkB beta (phospho T19)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human IkB beta around the phosphorylation site of threonine 19

  • General notes


    Detects IkB beta only when phosphorylated at threonine 19.

Properties

Applications

Our Abpromise guarantee covers the use of ab63510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/5000.

Target

  • FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • Tissue specificityExpressed in all tissues examined.
  • Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications
    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B beta antibody
    • I-kappa-B-beta antibody
    • IkappaBbeta antibody
    • IKB beta antibody
    • IkB-B antibody
    • IkB-beta antibody
    • IKBB antibody
    • IKBB_HUMAN antibody
    • IkBbeta antibody
    • NF kappa BIB antibody
    • NF-kappa-B inhibitor beta antibody
    • NF-kappa-BIB antibody
    • Nfkbib antibody
    • Thyroid receptor interacting protein 9 antibody
    • Thyroid receptor-interacting protein 9 antibody
    • TR interacting protein 9 antibody
    • TR-interacting protein 9 antibody
    • TRIP-9 antibody
    • TRIP9 antibody
    see all

References for Anti-IkB beta (phospho T19) antibody (ab63510)

ab63510 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I heard back from the lab that the immunogen specificity is for T19. Swiss Prot (http://www.uniprot.org/uniprot/Q15653#ref1) also mentions a sequence conflict, based on experimental info, that marks 19: S -> T (...

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