Anti-IKB epsilon (phospho S22) antibody (ab75907)

Overview

  • Product name
    Anti-IKB epsilon (phospho S22) antibody
    See all IKB epsilon primary antibodies
  • Description
    Rabbit polyclonal to IKB epsilon (phospho S22)
  • Specificity
    ab75907 detects endogenous levels of IKB epsilon only when phosphorylated at serine 22.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human IKB epsilon around the phosphorylation site of serine 22 (I-E-SP-L-R).

  • Positive control
    • Human breast carcinoma tissue; 293 cell extracts treated with TNF-alpha (20ng/ml, 15min).

Properties

Applications

Our Abpromise guarantee covers the use of ab75907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
  • Tissue specificity
    Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
  • Sequence similarities
    Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications
    Serine phosphorylated; followed by proteasome-dependent degradation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B epsilon antibody
    • I-kappa-B-epsilon antibody
    • IkappaBepsilon antibody
    • IkB E antibody
    • IkB-E antibody
    • IkB-epsilon antibody
    • IKBE antibody
    • IKBE_HUMAN antibody
    • MGC72568 antibody
    • NF kappa B inhibitor epsilon antibody
    • NF kappa BIE antibody
    • NF-kappa-B inhibitor epsilon antibody
    • NF-kappa-BIE antibody
    • NFkappa BIE antibody
    • NFkappaB inhibitor epsilon antibody
    • NFKBIE antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody
    • OTTHUMP00000016522 antibody
    • Slc35b2 antibody
    • solute carrier family 35, member B2 antibody
    see all

Images

  • ab75907 at 1/50 dilution staining IKB epsilon in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-IKB epsilon (phospho S22) antibody (ab75907) at 1/500 dilution

    Lane 1 : 293 cell extracts, untreated
    Lane 2 : 293 cell extracts, treated with TNF-alpha (20ng/ml, 15min)


    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References

ab75907 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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