Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

Overview

  • Product nameAnti-IKK alpha + IKK beta (phospho S180 + S181) antibodySee all IKK alpha + IKK beta primary antibodies ...
  • Description
    Rabbit polyclonal to IKK alpha + IKK beta (phospho S180 + S181)
  • SpecificityThis antibody detects endogenous levels of IKK alpha/beta ONLY when phosphorylated at serine 180/181.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of serine 180/181 (C-T-SP-F-V).

Properties

Applications

Our Abpromise guarantee covers the use of ab55341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA 1/20000.
IHC-P Use at an assay dependent dilution.

Target

  • RelevanceIKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
  • Cellular localizationCytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
  • Database links
  • Alternative names
    • CHUK antibody
    • Conserved helix loop helix ubiquitous kinase antibody
    • I kappa B kinase 1 antibody
    • I kappa B kinase 2 antibody
    • I Kappa B kinase alpha antibody
    • I Kappa B kinase beta antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IkBKB antibody
    • IKK a kinase antibody
    • IKK alpha antibody
    • IKK beta antibody
    • IKK1 antibody
    • IKK2 antibody
    • IKKA antibody
    • IKKB antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKA antibody
    • NFKBIKB antibody
    • Nuclear factor NF kappa B inhibitor kinase beta antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • TCF 16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody images

  • ab55341 at 1/100 staining human heart skeletal muscle sections (paraffin-embedded) by IHC-P. The image on the right has been treated with the synthesized phosphopeptide, image on the left is untreated.
  • ab55341 at 1/100 staining human heart (paraffin-embedded) using IHC. Image on right is treated with the synthesized phosphopeptide and the image on the left is untreated.
  • All lanes : Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

    Lane 1 : HepG2 cells treated with TNF (20 ng/ml, 5 mins)
    Lane 2 : HepG2 cells treated with TNF (20 ng/ml, 5 mins) with synthesized peptide

References for Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

ab55341 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (MCF-7 cells)
Loading amount 50 µg
Specification MCF-7 cells
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Submitted Apr 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"