Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

Overview

  • Product nameAnti-IKK alpha + IKK beta (phospho S180 + S181) antibodySee all IKK alpha + IKK beta primary antibodies ...
  • Description
    Rabbit polyclonal to IKK alpha + IKK beta (phospho S180 + S181)
  • SpecificityThis antibody detects endogenous levels of IKK alpha/beta ONLY when phosphorylated at serine 180/181.
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of serine 180/181 (C-T-SP-F-V).

Properties

Applications

Our Abpromise guarantee covers the use of ab55341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use at an assay dependent dilution.
ELISA 1/20000.
IHC-P Use at an assay dependent dilution.

Target

Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody images

  • ab55341 at 1/100 staining human heart skeletal muscle sections (paraffin-embedded) by IHC-P. The image on the right has been treated with the synthesized phosphopeptide, image on the left is untreated.
  • ab55341 at 1/100 staining human heart (paraffin-embedded) using IHC. Image on right is treated with the synthesized phosphopeptide and the image on the left is untreated.
  • All lanes : Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

    Lane 1 : HepG2 cells treated with TNF (20 ng/ml, 5 mins)
    Lane 2 : HepG2 cells treated with TNF (20 ng/ml, 5 mins) with synthesized peptide

References for Anti-IKK alpha + IKK beta (phospho S180 + S181) antibody (ab55341)

ab55341 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rabbit Cell lysate - whole cell (MCF-7 cells)
Loading amount 50 µg
Specification MCF-7 cells
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Submitted Apr 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"