Anti-IKK alpha + IKK beta antibody (ab63537)

Overview

  • Product nameAnti-IKK alpha + IKK beta antibody
    See all IKK alpha + IKK beta primary antibodies
  • Description
    Rabbit polyclonal to IKK alpha + IKK beta
  • SpecificityThis antibody detects endogenous levels of total IKK alpha and IKK beta protein.
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IKK alpha + IKK beta.

  • Positive control
    • Jurkat cell lysate or human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab63537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • RelevanceIKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
  • Cellular localizationCytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
  • Database links
  • Alternative names
    • CHUK antibody
    • Conserved helix loop helix ubiquitous kinase antibody
    • I kappa B kinase 1 antibody
    • I kappa B kinase 2 antibody
    • I Kappa B kinase alpha antibody
    • I Kappa B kinase beta antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IkBKB antibody
    • IKK a kinase antibody
    • IKK alpha antibody
    • IKK beta antibody
    • IKK1 antibody
    • IKK2 antibody
    • IKKA antibody
    • IKKB antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKA antibody
    • NFKBIKB antibody
    • Nuclear factor NF kappa B inhibitor kinase beta antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • TCF 16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Anti-IKK alpha + IKK beta antibody images

  • All lanes : Anti-IKK alpha + IKK beta antibody (ab63537) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells (5-30ug), without immunising peptide.
    Lane 2 : Extracts from Jurkat cells (5-30ug), with immunising peptide (5-10ug).


    Predicted band size : 85 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Ab63537 (1/50-1/100) staining human IKK alpha/IKK beta in human skeletal muscle tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.

References for Anti-IKK alpha + IKK beta antibody (ab63537)

ab63537 has not yet been referenced specifically in any publications.

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