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Rabbit polyclonal to IKK alpha + IKK beta
This antibody detects endogenous levels of total IKK alpha and IKK beta
Synthetic peptide corresponding to Human IKK alpha + IKK beta.
Jurkat cell lysate or human skeletal muscle tissue.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 85 kDa).
1/50 - 1/100.
IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
Cytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
Conserved helix loop helix ubiquitous kinase antibody
I kappa B kinase 1 antibody
Western blot - Anti-IKK alpha + IKK beta antibody (ab63537)
All lanes :
Anti-IKK alpha + IKK beta antibody (ab63537) at 1/500 dilution
Lane 1 :
Extracts from Jurkat cells (5-30ug), without immunising peptide.
Lane 2 :
Extracts from Jurkat cells (5-30ug), with immunising peptide (5-10ug).
Predicted band size :
Observed band size :
80 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK alpha + IKK beta antibody (ab63537)
Ab63537 (1/50-1/100) staining human IKK alpha/IKK beta in human skeletal muscle tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"