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Synthetic phosphopeptide derived from Human IKK alpha around the phosphorylation site of threonine 23 (LGTpGG).
Our Abpromise guarantee covers the use of ab38515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. See Abreview.|
Left image : untreated.
Right image : treated with phosphopeptide.
ab38515 at 1/100 staining mouse skin tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor ® conjugated goat anti-rabbit antibody was used as the secondary.