Overview

  • Product nameAnti-IKK beta antibody [10AG2]
    See all IKK beta primary antibodies
  • Description
    Mouse monoclonal [10AG2] to IKK beta
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody was raised against the 6xHis tag full-length protein corresponding to human IKK beta.

  • Positive control
    • Daudi whole cell extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20577263
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 90.7 kDa).

Target

  • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
  • Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
    Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
    Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
  • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase 2 antibody
    • I kappa B kinase beta antibody
    • I-kappa-B kinase 2 antibody
    • I-kappa-B-kinase beta antibody
    • IkBKB antibody
    • IKK beta antibody
    • IKK-B antibody
    • IKK-beta antibody
    • IKK2 antibody
    • IKKB antibody
    • IKKB_HUMAN antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKB antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    see all

Anti-IKK beta antibody [10AG2] images



  • Predicted band size : 90.7 kDa


    IKK beta detection by Western blot analysis. IKK beta can be detected in 30 µg of total cell extract from Daudi cells with ab12139 at a 1 µg/ml dilution.

References for Anti-IKK beta antibody [10AG2] (ab12139)

This product has been referenced in:

See all 5 Publications for this product

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