Overview

  • Product nameAnti-IKK beta antibody
    See all IKK beta primary antibodies
  • Description
    Rabbit polyclonal to IKK beta
  • Specificityab55404 detects endogenous levels of total IKK beta protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human IKK beta around the phosphorylation site of tyrosine 188 (L-Q-Y-L-A).

  • Positive control
    • Human breast carcinoma tissue and 293 cell extract, treated with LPS (100ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab55404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
  • Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
    Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
    Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
  • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase 2 antibody
    • I kappa B kinase beta antibody
    • I-kappa-B kinase 2 antibody
    • I-kappa-B-kinase beta antibody
    • IkBKB antibody
    • IKK beta antibody
    • IKK-B antibody
    • IKK-beta antibody
    • IKK2 antibody
    • IKKB antibody
    • IKKB_HUMAN antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKB antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    see all

Anti-IKK beta antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55404 (1/50 dilution), in the presence or absence of the immunizing peptide.
  • All lanes : Anti-IKK beta antibody (ab55404) at 1/500 dilution

    Lane 1 : 293 cell extract, treated with LPS (100ng/ml, 30mins), without the immunizing peptide
    Lane 2 : 293 cell extract, treated with LPS (100ng/ml, 30mins), with the immunizing peptide


    Predicted band size : 87 kDa
    Observed band size : 87 kDa
  • Anti-IKK beta antibody (ab55404) at 1/1000 dilution + Recombinant Human IKK beta protein (ab114243) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-IKK beta antibody (ab55404)

This product has been referenced in:
  • Londhe VA  et al. Conditional deletion of epithelial IKKß impairs alveolar formation through apoptosis and decreased VEGF expression during early mouse lung morphogenesis. Respir Res 12:134 (2011). IHC-P ; Mouse . Read more (PubMed: 21985298) »
  • Hou Y  et al. Analysis of the IKKbeta/NF-kappaB signaling pathway during embryonic angiogenesis. Dev Dyn 237:2926-35 (2008). IHC-P ; Mouse . Read more (PubMed: 18816823) »

See all 2 Publications for this product

Product Wall

I am pleased to inform you that the antibody recognizes all IKK Beta. The immunogen sequence is between the site 170 and 210 of IKK Beta. Please do not hesitate to contact me if you need further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"