Anti-IKK beta antibody [EPR6043] (ab124957)


  • Product nameAnti-IKK beta antibody [EPR6043]
    See all IKK beta primary antibodies
  • Description
    Rabbit monoclonal [EPR6043] to IKK beta
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues at the N terminal of Human IKK beta.

  • Positive control
    • HeLa and 293T cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab124957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).Can be blocked with IKK beta peptide (ab154148).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Anti-IKK beta antibody [EPR6043] images

    • Predicted band size : 87 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-IKK beta antibody [EPR6043] (ab124957) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 87 kDa
    • ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry.
    • ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry.
    • ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-IKK beta antibody [EPR6043] (ab124957)

    ab124957 has not yet been referenced specifically in any publications.

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