Validated using a knockout cell line
Recombinant
RabMAb

Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

Overview

  • Product name
    Anti-IKK beta antibody [EPR6043] - BSA and Azide free
    See all IKK beta primary antibodies
  • Description
    Rabbit monoclonal [EPR6043] to IKK beta - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • General notes

    ab171364 is a PBS-buffered version of ab124957, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab124957 for information on protocols, dilutions, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab171364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).

Can be blocked with ab154148

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(Heat to 98°C, allow to cool for 10-20 minutes)

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • Tissue specificity
      Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • Cellular localization
      Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Images

    • This WB data was generated using the same anti-IKK beta antibody clone [EPR6043] in a different buffer formulation (cat# ab124957).

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IKK beta knockout HAP1 cell lysate (20 µg) 
      Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    References

    ab171364 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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