Anti-IKK beta antibody [Y466] (HRP) (ab194713)


  • Product name
    Anti-IKK beta antibody [Y466] (HRP)
    See all IKK beta primary antibodies
  • Description
    Rabbit monoclonal [Y466] to IKK beta (HRP)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to residues near the C-term of human IKK-beta.

  • Positive control
    • WB: Daudi cell lysate and human cervical carcinoma. IHC-P: FFPE human breast adenocarcinoma.
  • General notes

    Alternative versions available:
    Anti-IKK beta antibody [Y466] (ab32135) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab194713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).


  • Function
    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
  • Tissue specificity
    Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
    Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
    Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
  • Cellular localization
    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase 2 antibody
    • I kappa B kinase beta antibody
    • I-kappa-B kinase 2 antibody
    • I-kappa-B-kinase beta antibody
    • IkBKB antibody
    • IKK beta antibody
    • IKK-B antibody
    • IKK-beta antibody
    • IKK2 antibody
    • IKKB antibody
    • IKKB_HUMAN antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKB antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    see all


  • Anti-IKK beta antibody [Y466] (HRP) (ab194713) at 1/5000 dilution + Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 87 kDa

    Exposure time: 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% milk before being incubated with ab194713 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.


ab194713 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194713.
Please use the links above to contact us or submit feedback about this product.


Sign up