Anti-IKK beta (phospho Y188) antibody (ab194519)
Key features and details
- Rabbit polyclonal to IKK beta (phospho Y188)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-IKK beta (phospho Y188) antibody
See all IKK beta primary antibodies -
Description
Rabbit polyclonal to IKK beta (phospho Y188) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human IKK beta (phospho Y188) conjugated to keyhole limpet haemocyanin.
Database link: O14920 -
Positive control
- WB: COS7 cell extract. IHC-P: Human breast cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab194519 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 87 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 87 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. -
Tissue specificity
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUpon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
Cellular localization
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
see all -
Alternative names
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue staining IKK beta (phospho Y188) using ab194519 at 1:100 dilution (4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive control (right) obtained from antibody was pre-absorbed by immunogen peptide.
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All lanes : Anti-IKK beta (phospho Y188) antibody (ab194519) at 1/500 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with antigen-specific peptide
Predicted band size: 87 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab194519 has been referenced in 9 publications.
- Lang GP et al. Rutin pretreatment promotes microglial M1 to M2 phenotype polarization. Neural Regen Res 16:2499-2504 (2021). PubMed: 33907040
- Sun X et al. The Nrf2 activator RTA-408 attenuates osteoclastogenesis by inhibiting STING dependent NF-?b signaling. Redox Biol 28:101309 (2020). PubMed: 31487581
- Dong Y et al. Maternally-Expressed Gene 3 (MEG3)/miR-143-3p Regulates Injury to Periodontal Ligament Cells by Mediating the AKT/Inhibitory ?B Kinase (IKK) Pathway. Med Sci Monit 26:e922486 (2020). PubMed: 32520926
- Jiang L et al. Overexpression of PIMREG promotes breast cancer aggressiveness via constitutive activation of NF-?B signaling. EBioMedicine 43:188-200 (2019). PubMed: 30979686
- Liu B et al. IKKß regulates the expression of coagulation and fibrinolysis factors through the NF-?B canonical pathway in LPS-stimulated alveolar epithelial cells type II. Exp Ther Med 18:2859-2866 (2019). PubMed: 31572531
- Mei L et al. Paeonol attenuates inflammation by targeting HMGB1 through upregulating miR-339-5p. Sci Rep 9:19370 (2019). PubMed: 31852965
- Liu X et al. Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling. Cell Res 29:42-53 (2019). PubMed: 30514903
- Jiang Y et al. TIPE2 governs macrophage polarization via negative regulation of mTORC1. Mol Med Rep 17:952-960 (2018). PubMed: 29115630
- Porteiro B et al. Hepatic p63 regulates steatosis via IKKß/ER stress. Nat Commun 8:15111 (2017). WB ; Mouse . PubMed: 28480888