Overview

  • Product nameAnti-IKK beta (phospho Y199) antibody
    See all IKK beta primary antibodies
  • Description
    Rabbit polyclonal to IKK beta (phospho Y199)
  • SpecificityDetects endogenous levels of IKK beta only when phosphorylated at tyrosine 199.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from around the phosphorylation site of tyrosine 199 (QKYPTV) of human IKK beta

  • Positive control
    • Human breast carcinoma tissue HeLa cells treated with TNF-alpha and calyculin

Properties

Applications

Our Abpromise guarantee covers the use of ab59195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Anti-IKK beta (phospho Y199) antibody images

    • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59195 at 1/50 dilution. Left image: untreated. Right image: sample treated with immunising peptide.
    • All lanes : Anti-IKK beta (phospho Y199) antibody (ab59195) at 1/500 dilution

      Lane 1 : HeLa cell extracts treated with TNF alpha (20ng/ml) and calyculin (50nM, 5 mins)
      Lane 2 : HeLa cell extracts treated with TNF alpha (20ng/ml) and calyculin (50nM, 5 mins) with phosphopeptide


      Predicted band size : 87 kDa
      Observed band size : 87 kDa

    References for Anti-IKK beta (phospho Y199) antibody (ab59195)

    This product has been referenced in:
    • Fang L  et al. ATM regulates NF-?B-dependent immediate-early genes via RelA Ser 276 phosphorylation coupled to CDK9 promoter recruitment. Nucleic Acids Res N/A:N/A (2014). Read more (PubMed: 24957606) »
    • Wei W  et al. The breast cancer susceptibility gene product fibroblast growth factor receptor 2 serves as a scaffold for regulation of NF-?B signaling. Mol Cell Biol 32:4662-73 (2012). WB . Read more (PubMed: 22988296) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (macrophage)
    Specification macrophage
    Treatment 0.1 ug/ml LPS 15 min
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (prostate adenocarcinoma)
    Specification prostate adenocarcinoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA (pH 9)
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Lewis MacKenzie

    Verified customer

    Submitted Feb 12 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"