Overview

  • Product nameAnti-IKKi/IKKe antibody
    See all IKKi/IKKe primary antibodies
  • Description
    Rabbit polyclonal to IKKi/IKKe
  • SpecificityRecognises IKK epsilon and does not cross react with IKK alpha, beta or gamma.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Peptide (

    NRIIERLNRVPAPPDV

    ) corresponding to aminoacids 701 to 716 of human IKKe/IKK-i (1,2).

  • Positive control
    • Whole cell lysate from Jurkat cells
  • General notes


    Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS.

Properties

Applications

Our Abpromise guarantee covers the use of ab7891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
IP Use a concentration of 3 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.

Target

  • FunctionPhosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
  • Tissue specificityHighly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
  • Cellular localizationCytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase epsilon antibody
    • I-kappa-B kinase epsilon antibody
    • IkBKE antibody
    • IKK related kinase epsilon antibody
    • IKK-E antibody
    • IKK-epsilon antibody
    • IKK-i antibody
    • IKKE antibody
    • IKKE_HUMAN antibody
    • IKKepsilon antibody
    • IKKI antibody
    • Inducible I kappa B kinase antibody
    • Inducible I kappa-B kinase antibody
    • Inducible IkappaB kinase antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
    • KIAA0151 antibody
    • MGC125294 antibody
    • MGC125295 antibody
    • MGC125297 antibody
    see all

Anti-IKKi/IKKe antibody images

  • Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
  • ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC

  • ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-IKKi/IKKe antibody (ab7891)

This product has been referenced in:
  • Gu L  et al. Human DEAD box helicase 3 couples I?B kinase e to interferon regulatory factor 3 activation. Mol Cell Biol 33:2004-15 (2013). Read more (PubMed: 23478265) »
  • Paladino P  et al. Cellular localization of the herpes simplex virus ICP0 protein dictates its ability to block IRF3-mediated innate immune responses. PLoS One 5:e10428 (2010). WB ; Human . Read more (PubMed: 20454685) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 15 µg
Specification MCF7
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Aug 12 2009

This antibody has not yet been tested with mouse ikk epsilon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"