Anti-IKKi/IKKe antibody - Aminoterminal end (ab70847)

Overview

  • Product name
    Anti-IKKi/IKKe antibody - Aminoterminal end
    See all IKKi/IKKe primary antibodies
  • Description
    Rabbit polyclonal to IKKi/IKKe - Aminoterminal end
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the N-terminal region of human IKKE conjugated to KLH

  • Positive control
    • THP-1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

Target

  • Function
    Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
  • Tissue specificity
    Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase epsilon antibody
    • I-kappa-B kinase epsilon antibody
    • IkBKE antibody
    • IKK related kinase epsilon antibody
    • IKK-E antibody
    • IKK-epsilon antibody
    • IKK-i antibody
    • IKKE antibody
    • IKKE_HUMAN antibody
    • IKKepsilon antibody
    • IKKI antibody
    • Inducible I kappa B kinase antibody
    • Inducible I kappa-B kinase antibody
    • Inducible IkappaB kinase antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
    • KIAA0151 antibody
    • MGC125294 antibody
    • MGC125295 antibody
    • MGC125297 antibody
    see all

Images

  • Anti-IKKi/IKKe antibody - Aminoterminal end (ab70847) at 1/100 dilution + THP-1 cell lysate. at 12.5 µg

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,23 kDa,30 kDa,48 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References

ab70847 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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