Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

Overview

  • Product nameAnti-IKKi/IKKe antibody [EPR4904]
    See all IKKi/IKKe primary antibodies
  • Description
    Rabbit monoclonal [EPR4904] to IKKi/IKKe
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human IKKi/IKKe.

  • Positive control
    • HeLa, Raji, MCF7 and Jurkat cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionPhosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
    • Tissue specificityHighly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
      Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
    • Cellular localizationCytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
    • Information by UniProt
    • Database links
    • Alternative names
      • I kappa B kinase epsilon antibody
      • I-kappa-B kinase epsilon antibody
      • IkBKE antibody
      • IKK related kinase epsilon antibody
      • IKK-E antibody
      • IKK-epsilon antibody
      • IKK-i antibody
      • IKKE antibody
      • IKKE_HUMAN antibody
      • IKKepsilon antibody
      • IKKI antibody
      • Inducible I kappa B kinase antibody
      • Inducible I kappa-B kinase antibody
      • Inducible IkappaB kinase antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
      • KIAA0151 antibody
      • MGC125294 antibody
      • MGC125295 antibody
      • MGC125297 antibody
      see all

    Anti-IKKi/IKKe antibody [EPR4904] images

    • All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 80 kDa
    • ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

    ab124766 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124766.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"