Overview

  • Product name
  • Description
    Rabbit polyclonal to IKZF5
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Fetal muscle lysate, human skin

Properties

Applications

Our Abpromise guarantee covers the use of ab42469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    DNA-binding protein that binds to the 5'GNNTGTNG-3' core sequence. Transcriptional repressor.
  • Tissue specificity
    Expressed in brain, heart, skeletal muscle, kidney, and liver. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
  • Sequence similarities
    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain
    The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
    C-terminal zinc fingers mediate homodimerization.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781B0249 antibody
    • FLJ22973 antibody
    • IKAROS family zinc finger 5 (Pegasus) antibody
    • Ikaros family zinc finger protein 5 antibody
    • IKZF5 antibody
    • IKZF5_HUMAN antibody
    • PEGASUS antibody
    • Zinc finger protein Pegasus antibody
    • Zinc finger protein subfamily 1A 5 antibody
    • Zinc finger transcription factor Pegasus antibody
    • ZNFN1A5 antibody
    see all

Images

  • Anti-IKZF5 antibody (ab42469) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
  • ab42469 at 8.0µg/ml staining human skin for IKZF5 expression. Arrows indicate staining of squamous epithelial cells.

References

ab42469 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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