IL-12 p40 (Interleukin-40) Human ELISA Kit (ab113324)

Overview

  • Product nameIL-12 p40 (Interleukin-40) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    12.29 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 127.6 120% - 134%
    Serum 99.73 91% - 108%
    Plasma 91.11 85% - 98%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 P40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 P40 in serum (Human IL-12 p40 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-12 P40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 P40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 P40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 P40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B Item E 1 x 15ml
    Assay Diluent C Item L 1 x 30ml
    Biotinylated anti-Human IL-12 P40 Item F 2 vials
    IL-12 P40 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-12 P40 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Involvement in diseaseDefects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P29460 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • 40 kD Subunit
    • CLMF
    • CLMF 2
    • CLMF p40
    • CLMF p40
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 2
    • Cytotoxic lymphocyte maturation factor 2 p40
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • HGNC:5970
    • IL 12 subunit p40
    • IL 12B
    • IL-12 subunit p40
    • IL-12 subunit p40
    • IL-12B
    • IL-12B
    • IL12 subunit p40
    • IL12B
    • IL12B
    • IL12B_HUMAN
    • IL12B_HUMAN
    • Interleukin 12 beta chain
    • Interleukin 12 p40
    • Interleukin 12 subunit beta
    • Interleukin 12B
    • Interleukin-12 subunit beta
    • Interleukin-12 subunit beta
    • Interleukin12B
    • Natural killer cell stimulatory factor 2
    • Natural killer cell stimulatory factor 40 kD subunit
    • NK cell stimulatory factor chain 2
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF 2
    • NKSF2
    • NKSF2
    • p40
    • Subunit P40
    • T cell stimulating factor
    • TcMF
    • TSF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

IL-12 p40 (Interleukin-40) Human ELISA Kit images

  • Representative standard curve using ab113324

  • Representative standard curve using ab113324

Protocols

References for IL-12 p40 (Interleukin-40) Human ELISA Kit (ab113324)

ab113324 has not yet been referenced specifically in any publications.

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