IL-12 p70 (Interleukin-12 p70) Human ELISA kit (ab119532)

Overview

  • Product nameIL-12 p70 (Interleukin-12 p70) Human ELISA kit
    See all IL12 p70 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3%
    Overall 8 3%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    2.1 pg/ml
  • Range
    3.1 pg/ml - 200 pg/ml
  • Recovery

    103 %

    Sample specific recovery
    Sample type Average % Range
    Serum 103 79% - 118%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 p70 concentrations in Cell culture supernatant serum, and plasma (EDTA, citrate, heparin).

    Human IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 p70 captured on the plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human IL12 p70 monoclonal antibody 1 x 100µl
    Blue-Dye 1 x 400µl
    Control high 1 vial
    Control low 1 vial
    Green-Dye 1 x 400µl
    IL-12 p70 Standard, lyophilized (400 pg/mL on reconstitution) 2 vials
    Microplate coated with antibody to Human IL-12 p70 (12 x 8 wells) 1 unit
    Red-Dye 1 x 400µl
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research Areas
  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

IL-12 p70 (Interleukin-12 p70) Human ELISA kit images

  • Representative Standard Curve using ab119532.

Protocols

References for IL-12 p70 (Interleukin-12 p70) Human ELISA kit (ab119532)

ab119532 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119532.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"