Overview

  • Product nameIL-17 (Interleukin -17)  Human ELISA Kit
    See all IL17 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    3.12 pg/ml - 100 pg/ml
  • Recovery

    105 %

    Sample specific recovery
    Sample type Average % Range
    Serum 121 113% - 130%

  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-17 (Interleukin-17) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17 in supernatants, buffered solutions, serum, plasma and other body fluids.

    A monoclonal antibody specific for IL-17 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-17.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificityRestricted to activated memory T-cells.
  • Sequence similaritiesBelongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab83688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

IL-17 (Interleukin -17)  Human ELISA Kit images

  • IL-17 measured in cell culture supernatants from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).

  • Representative Standard Curve using ab83688

Protocols

References for IL-17 (Interleukin -17)  Human ELISA Kit (ab83688)

This product has been referenced in:
  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"