IL-28 (Interleukin-28) Mouse ELISA Kit (ab100708)
- Product nameIL-28 (Interleukin-28) Mouse ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 2 pg/ml
- Range2.46 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 74.3 67% - 87% Serum 109.8 98% - 118% Plasma 76.8 67% - 93%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse IL-28 2 vials IL-28 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-28 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Research Areas
- Cytokine Zcyto20
- IFN lambda 2
- Interferon lambda 2
- Interleukin 28A
Our Abpromise guarantee covers the use of ab100708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IL-28 (Interleukin-28) Mouse ELISA Kit (ab100708)
ab100708 has not yet been referenced specifically in any publications.