IL-6 (Interleukin-6) Rat ELISA Kit (ab119548)

Overview

  • Product nameIL-6 (Interleukin-6) Rat ELISA Kit
    See all IL6 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6 < 5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    12 pg/ml
  • Range
    31 pg/ml - 2000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-6 (Interleukin-6) rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-6 in culture supernatant and serum.

    Il-6 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Il-6 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic an stop solution. The density of yellow coloration is directly proportional to the amount of Il-6 captured on the plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin BSF2
    • Interleukin-6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

IL-6 (Interleukin-6) Rat ELISA Kit images

  • Representative Standard Curve using ab119548.

Protocols

References for IL-6 (Interleukin-6) Rat ELISA Kit (ab119548)

ab119548 has not yet been referenced specifically in any publications.

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