Overview

  • Product name
  • Description
    Goat polyclonal to IL1 alpha
  • Tested applications
    Suitable for: WB, Sandwich ELISA, Neutralising, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rIL-1a (rat Interleukin-1-alpha).

Properties

Applications

Our Abpromise guarantee covers the use of ab9875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to IL1 alpha (Biotin) (ab84012). Using ab84012 as the detection antibody and ab109792 as the capture antibody, the detection limit for recombinant Rat IL1 alpha is 0.2 - 0.4 ng/well
Neutralising Use a concentration of 0.011 - 0.017 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat IL1 alpha (50 pg/ml).
IHC-FoFr Use at an assay dependent dilution.

Target

  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all

Anti-IL1 alpha antibody images

  • Immunohistochemical analysis of rat brain tissue, staining IL1 alpha in microglia, with ab9875 (1/1000). An AlexaFluor®-conjugated anti-goat IgG was used as the secondary antibody.
  • Immunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining IL1 alpha with ab9875 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.
  • Immunohistochemical analysis of PFA perfusion fixed frozen rat brain, staining IL1 alpha with ab9875 at 1 µg/ml. A peroxidase conjugated secondary antibody was added and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.

References for Anti-IL1 alpha antibody (ab9875)

This product has been referenced in:
  • Johnson EA & Kan RK The acute phase response and soman-induced status epilepticus: temporal, regional and cellular changes in rat brain cytokine concentrations. J Neuroinflammation 7:40 (2010). IHC-FoFr ; Rat . Read more (PubMed: 20649973) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Fibrotic Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization
No
Specification
Fibrotic Skin
Blocking step
Serum Free Protein Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted May 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Inflamed Spinal Cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
Permeabilization
No
Specification
Inflamed Spinal Cord
Blocking step
Serum Free Protein Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted May 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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