Anti-IL1 alpha antibody [AS5] (ab17281)

Overview

  • Product nameAnti-IL1 alpha antibody [AS5]
    See all IL1 alpha primary antibodies
  • Description
    Mouse monoclonal [AS5] to IL1 alpha
  • SpecificityRecognizes human IL-1 alpha. Does not recognize mouse IL-1 alpha, human IL-1 beta or a panel of other human cytokines.
  • Tested applicationsSuitable for: IHC-P, IP, ELISA, Neutralising, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant IL1 alpha protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab17281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. (see Abreview)
IP Use at an assay dependent concentration. This antibody has been used to immunoprecipitate >90% human IL-1 (in solution using a double-antibody method).
ELISA Use a concentration of 2 µg/ml. This antibody has been used as a solution phase antibody in a two site ELISA with biotin conjugated IL-1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human IL-1 alpha in human serum is typically >85% in the ELISA.
Neutralising Use a concentration of 25 mg/ml. This antibody has been used to neutralize >95% of the biological activity of human IL-1 at 0.5 mg/ml.
IHC (PFA fixed) Use a concentration of 1 - 10 µg/ml. This antibody has been used to stain human IL-1 alpha expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4oC plus 0.1% saponin 20 minutes at RT.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all

Anti-IL1 alpha antibody [AS5] images

  • ab17281 staining IL1 alpah in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes at 21°C. A undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References for Anti-IL1 alpha antibody [AS5] (ab17281)

ab17281 has not yet been referenced specifically in any publications.

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Thanks for your enquiry.

Its estimated we will havemore vialsof ab17281 in stock by Monday 6, 2012.

I hope this is helpful. Please contact us again if you have any further questions.

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A new vial of ab17281 is on its way to you, Your new order number if *.

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Thank you for your reply.

Unfortunately we do not have any other lots of this antibody available, so maybe it would be better to send a different IL-1 alpha antibody for you try.Or if you wish to give ab17281 another try, I’m happy to se...

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Thanks for letting me know.

I am sorry that increasing the primary antibody concentration did not work for you. Therefore I think the best way to move forward on this one, is to either send you a new aliquot of ab17281, in case there was somet...

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I am sorry about the issues you have been having with ab17281 in IHC-P. If the protocol variations we talked about today do not prove to be successful, then please let me know as the antibody is covered under ou...

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Thank you once again for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investig...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
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Submitted Jan 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"