Anti-IL1 alpha antibody [AS6] (Biotin) (ab16163)

Overview

  • Product name
    Anti-IL1 alpha antibody [AS6] (Biotin)
    See all IL1 alpha primary antibodies
  • Description
    Mouse monoclonal [AS6] to IL1 alpha (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Does not recognise human IL1 beta or a panel of other human cytokines
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

Applications

Our Abpromise guarantee covers the use of ab16163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: This antibody has been used as a solution phase antibody at 2 µg/ml in a two site ELISA with unconjugated IL1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5pg/ml with the appropriate standard.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
    • Sequence similarities
      Belongs to the IL-1 family.
    • Domain
      The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
    • Cellular localization
      Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAF antibody
      • FAF antibody
      • Hematopoietin 1 antibody
      • Hematopoietin-1 antibody
      • IL 1 alpha antibody
      • IL 1A antibody
      • IL-1 alpha antibody
      • Il-1a antibody
      • IL1 ALPHA antibody
      • IL1 antibody
      • IL1A antibody
      • IL1A_HUMAN antibody
      • IL1F1 antibody
      • Interleukin 1 alpha antibody
      • Interleukin-1 alpha antibody
      • Interleukin1 alpha antibody
      • LAF antibody
      • LEM antibody
      • Preinterleukin 1 alpha antibody
      • Pro interleukin 1 alpha antibody
      see all

    References

    ab16163 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    ELISA
    Sample
    Human Recombinant protein (Recombinant Human IL-1alpha from PeproTech)
    Specification
    Recombinant Human IL-1alpha from PeproTech
    Type
    Sandwich (Detection)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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