The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: This antibody has been used as a solution phase antibody at 2 µg/ml in a two site ELISA with unconjugated IL1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5pg/ml with the appropriate standard.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Belongs to the IL-1 family.
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.