Overview

  • Product nameAnti-IL1 beta antibody [11E5]See all IL1 beta primary antibodies ...
  • Description
    Mouse monoclonal [11E5] to IL1 beta
  • SpecificityDoes not inhibit the biological activity of Interleukin-1.
  • Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant beta human Interleukin-1

Properties

Applications

Our Abpromise guarantee covers the use of ab8320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [5C10] to IL1 beta (HRP) (ab8321).
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5C10] to IL1 beta (HRP) (ab8321). Can be used as Capture antibody with ab8321. For 2 site enzyme immunoassay, we recommend to use a mixture of this antibody and ab8319 for coating, in conjunction with ab8321 for labeling.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • Hematopoietin 1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL 1B antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • Interleukin-1 beta antibody
    • LAF antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody [11E5] images

  • ab8320 staining IL1 beta in lung inflammatory cells from Rhesus monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Tween 20 and blocked with 10% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/25 in PBS + 10% serum + 1% Tween 20) for 18 hour at 4°C. An diluted Alexa Fluor®594-conjugated + 1.4nm Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of frozen Human skin tissue taken from patients with localized cutaneous leishmaniasis (A, C), diffuse cutaneous leishmaniasis (E). Normal tissue (G) was used as a negative control.

    IL1 beta was stained using ab8320 at 1/100 dilution for 1 hour at room temperature. ab94705 Mouse and Rabbit Specific HRP/AEC Detection IHC Kit was used to detect staining. Hematoxylin was used as a counterstain.

References for Anti-IL1 beta antibody [11E5] (ab8320)

This product has been referenced in:
  • Fernández-Figueroa EA  et al. Disease severity in patients infected with Leishmania mexicana relates to IL-1ß. PLoS Negl Trop Dis 6:e1533 (2012). IHC-Fr ; Human . Read more (PubMed: 22629474) »
  • Meng CX  et al. Effects of oral and vaginal administration of levonorgestrel emergency contraception on markers of endometrial receptivity. Hum Reprod 25:874-83 (2010). Read more (PubMed: 20139427) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rhesus monkey Cell (Lung - inflammatory cells)
Specification Lung - inflammatory cells
Fixative Formaldehyde
Permeabilization Yes - Tween-20
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2013

I am glad that the Rhesus tissue worked well! Unfortunately the homology for ab8320 with sheep is only 60%, to low for any reactivity. Another IL1-beta antibody, ab45692 does have 88% homology but has only been tested for Western Blot. Please let me kn...

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Thank you very much for contacting us. To our knowledge, ab8320 has not been tested in rhesus monkey. The immunogen for this product is full length human IL1 beta, which has more than 90% homology with the Rhesus sequence. Based on this, I would predic...

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Gracias por contactarnos de nuevo.

Contáctanos tantas veces como sea necesario, no es ningún problema, estaré encantada de poder ayudar.

Para detectar IL1 beta por Sándwich ELISA, ab8320 es una buena opci...

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Thank you for your enquiry and your interest in our products. Recommended positive control for this antibody can be activated human lymphocytes. We hope this will help.

Thank you for your email. I would suggest starting with a range of dilutions, 1:50, 1:100, 1:200, and then optimize from there based on your results. If you have any more questions, please contact us again.

Yes, we this antibody is routinely used in this application, although we have not tested it ourselves.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"