• Product name
  • Description
    Rabbit polyclonal to IL1 beta
  • Specificity
    This antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected. The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein.
  • Tested applications
    Suitable for: WB, Neutralising, IHC-Fr, IHC-P, IHC-FoFr, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Rat IL1 beta aa 2-268.


    Database link: Q63264

  • Positive control
    • recombinant rat IL1 beta (lower limit of detection: 1.95 ng/lane)



Our Abpromise guarantee covers the use of ab9787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of IL1 beta (0.20 ng/ml), a concentration of 0.09 - 0.14 µg/ml of this antibody is required.

IHC-Fr Use at an assay dependent concentration. PubMed: 20187933
IHC-P Use at an assay dependent concentration. PubMed: 22315633
IHC-FoFr Use at an assay dependent concentration. PubMed: 20649973
Sandwich ELISA Use at an assay dependent concentration.

To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.


  • Function
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificity
    Expressed in activated monocytes/macrophages (at protein level).
  • Sequence similarities
    Belongs to the IL-1 family.
  • Post-translational
    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localization
    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody images

  • Immunohistochemical analysis of rat endometrial tissue, staining IL1 beta with ab9787.

    Tissue was taken from pregnant rats with implantation failure. Antigen retrieval was by heat mediation in a citrate buffer (pH 6) followed by blocking with 1% BSA for 30 minutes. Sections were incubated with primary antibody (1/50) overnight at 4°C. An HRP-goat anti-rabbit IgG was used as the secondary antibody (1/200). Staining was detected using DAB.
  • Immunohistochemical analysis of rat brain tissue, staining IL1 beta with ab9787.

    Tissue was taken from rats with middle cerebral artery occlusion either untreated (left) or treated with specific MEK1/2 inhibitor U0126. Samples were fixed for 10 minutes in ice-cold acetone and then rehydrated in PBS containing 0.3% Triton X-100 for 15 minutes. The tissues were then permeabilized and blocked for 1 hour in blocking solution containing PBS, 0.3% Triton X-100, 1% BSA, and 5% normal donkey serum. Samples were incubated overnight at 4°C with primary antibody (1/400) and a Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
  • All lanes : Anti-IL1 beta antibody (ab9787) at 0.2 µg/ml

    Lane 1 : MultiMark MultiColor Standard (Invitrogen)
    Lane 2 : 250 ng/lane
    Lane 3 : 125 ng/lane
    Lane 4 : 62.5 ng/lane
    Lane 5 : 31.25 ng/lane
    Lane 6 : 15.6 ng/lane
    Lane 7 : 7.8 ng/lane
    Lane 8 : 3.9 ng/lane
    Lane 9 : 1.95 ng/lane

    Observed band size : 17.3 kDa (why is the actual band size different from the predicted?)
    The lower detection limit of Ab9787 lies at approximately 1.95 ng.

References for Anti-IL1 beta antibody (ab9787)

This product has been referenced in:
  • Linden MA  et al. A return to ad libitum feeding following caloric restriction promotes hepatic steatosis in hyperphagic OLETF rats. Am J Physiol Gastrointest Liver Physiol 311:G387-95 (2016). Read more (PubMed: 27445343) »
  • Liu B  et al. Gastrodin ameliorates subacute phase cerebral ischemia-reperfusion injury by inhibiting inflammation and apoptosis in rats. Mol Med Rep 14:4144-4152 (2016). WB ; Rat . Read more (PubMed: 27748849) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Spinal cord)
Spinal cord
Antigen retrieval step
Yes - 0.3% Triton-X100 in blocking buffer
Blocking step
normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (Macrophages and dentritic cells)
Loading amount
15 µg
Macrophages and dentritic cells
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 05 2012

I am very pleased to hear you would like to accept our offer and test ab9787 in WB (endogenous protein). This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and inc...

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Thank you for your message.

My colleague Padamjeet is out of office today. I can confirm that the expiration date: 17-12-2012.

Apologiesfor this mistake. If you need any further assistance in the future, please do not hesitate to co...

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Expiration date: 17-08-2012

I am very pleased to hear you would like to accept our offer and test ab9787 in [Mouse]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem th...

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Thank you for your enquiry.

As far as we are aware, ab9722 IL1 beta antibodyhas been tested and guaranteed in mouse but nottested in rat. the other ab9787IL1 beta antibody is tested and guaranteed for rat but not tested in mouse.


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Thank you for your reply.

Unfortunately I am not sure what the identity of the extra bands might be. The protein you are using is not 100% pure so possibly other contaminants or strong multimers? It is reassuring that the antibody is reco...

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Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXX.

To check the s...

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Thank you for your patience as I have investigated this issue further with the laboratory.

Based on your results and those obtained in our own laboratory it is not clear why this antibody is detecting a 23kDa band in your samples. I am ha...

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Thank you for contacting Abcam regarding ab9787.

I have reviewed the data you sent and I was hoping you would be able to send me some more information regarding the experimental setup and WB protocol.

In particularwhat are y...

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