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Recombinant full length protein corresponding to Rat IL1 beta aa 2-268.
MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
Our Abpromise guarantee covers the use of ab9787 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Neutralising||Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of IL1 beta (0.20 ng/ml), a concentration of 0.09 - 0.14 µg/ml of this antibody is required.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20187933|
|IHC-P||Use at an assay dependent concentration. PubMed: 22315633|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20649973|
|Sandwich ELISA||Use at an assay dependent concentration.
To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.