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Recombinant full length protein corresponding to Rat IL1 beta aa 2-268.
MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
Our Abpromise guarantee covers the use of ab9787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Neutralising||Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of IL1 beta (0.20 ng/ml), a concentration of 0.09 - 0.14 µg/ml of this antibody is required.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20187933|
|IHC-P||Use at an assay dependent concentration. PubMed: 22315633|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20649973|
|Sandwich ELISA||Use at an assay dependent concentration.
To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.