Overview

  • Product nameAnti-IL1 beta antibody (HRP)
    See all IL1 beta primary antibodies
  • Description
    Rabbit polyclonal to IL1 beta (HRP)
  • ConjugationHRP
  • Specificityab106035 is primarily directed against mature, 17,000 MW Mouse IL1 beta. It will recognize 10% of the non-denatured (native) precursor 31,000 MW Mouse IL1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. ab106035 does not detect IL1 alpha.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IP, RIA, IHC-Fr, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Human, Non Human Primates
  • Immunogen

    Recombinant Mouse IL1 beta produced in E.coli. The MW of recombinant mouse IL1 beta was 17 kDa

  • Positive control
    • Rat and Mouse endotoxin stimulated peripheral blood mononuclear cells (PBMC).
  • General notesDo NOT add Sodium Azide!

Properties

Applications

Our Abpromise guarantee covers the use of ab106035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17, 30 kDa.
IHC-P 1/500 - 1/2500.
Flow Cyt Use at an assay dependent concentration. Caution should be exhibited as the F(c) domain of the Rabbit IgG molecule may interact with cells non-specifically.
IP Use at an assay dependent concentration. Preclearing the preparation with a non-specific Rabbit IgG to reduce background is suggested.
RIA Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/2500.
ELISA 1/10000 - 1/50000. Best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
Neutralising Use at an assay dependent concentration. It is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested.

Target

  • FunctionPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificityExpressed in activated monocytes/macrophages (at protein level).
  • Sequence similaritiesBelongs to the IL-1 family.
  • Post-translational
    modifications
    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localizationCytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

References for Anti-IL1 beta antibody (HRP) (ab106035)

This product has been referenced in:
  • Tang YL  et al. TLR4/NF-?B signaling contributes to chronic unpredictable mild stress-induced atherosclerosis in ApoE-/- mice. PLoS One 10:e0123685 (2015). WB ; Mouse . Read more (PubMed: 25860573) »

See 1 Publication for this product

Product Wall

Thank you for submitting an Abreview of ab106035. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

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Application Western blot
Sample Mouse Cell lysate - whole cell (macrophgaes cell line)
Loading amount 50 µg
Specification macrophgaes cell line
Treatment LPS
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 21 2012

Thank you for contacting us.

The anti-IL1 beta antibody (HRP) (ab106035) was raised against the full length mouse IL1 beta protein expressed in E. coli. This antibody has been validated in ELISA and would therefore be guaranteed by the Abprom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"