Anti-IL1 Receptor I antibody [40101] (ab115497)

Overview

  • Product nameAnti-IL1 Receptor I antibody [40101]
    See all IL1 Receptor I primary antibodies
  • Description
    Mouse monoclonal [40101] to IL1 Receptor I
  • Specificityab115497 reacts with cell surface and soluble human IL1 Receptor I. The specific epitope is unknown. ab115497 does not block binding of IL-1 to the receptor, and does not cross-react with receptors for human IL-2 or IL-4.
  • Tested applicationsSuitable for: ICC, ELISA, IHC-P, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human IL1 Receptor I.

  • Positive control
    • Human Placenta tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 - 100 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration. Concentrations of 20-30 ng/ml will bind radiolabeled IL1 Receptor I in a radioimmunoassay format.

Target

  • FunctionReceptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similaritiesBelongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

Anti-IL1 Receptor I antibody [40101] images

  • ab115497, at 10 µg/ml, staining IL1 Receptor I in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-IL1 Receptor I antibody [40101] (ab115497)

ab115497 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization No
Fixative Paraformaldehyde
Username

Ms. Michaela Blanfeld

Verified customer

Submitted Nov 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"