Anti-IL1 Receptor I antibody [40101] (ab115497)

Overview

  • Product name
    Anti-IL1 Receptor I antibody [40101]
    See all IL1 Receptor I primary antibodies
  • Description
    Mouse monoclonal [40101] to IL1 Receptor I
  • Specificity
    ab115497 reacts with cell surface and soluble human IL1 Receptor I. The specific epitope is unknown. ab115497 does not block binding of IL-1 to the receptor, and does not cross-react with receptors for human IL-2 or IL-4.
  • Tested applications
    Suitable for: ICC, ELISA, IHC-P, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human IL1 Receptor I.

  • Positive control
    • Human Placenta tissue.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab115497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 - 100 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration. Concentrations of 20-30 ng/ml will bind radiolabeled IL1 Receptor I in a radioimmunoassay format.

Target

  • Function
    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similarities
    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

Images

  • ab115497, at 10 µg/ml, staining IL1 Receptor I in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Ms. Michaela Blanfeld

Verified customer

Submitted Nov 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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