The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Ab87551, when used as detection antibody with recommended pair, can detect at least 0.2 – 0.4 ng/well of recombinant Human IL1 Receptor I.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 65 kDa. When used in conjunction with compatible secondary reagents, the detection limit for Ab87551 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.