The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 65 kDa.
Use a concentration of 5 µg/ml.
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling IL1 Receptor I with ab190078 at 5 µg/ml, followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot - Anti-IL1 Receptor I antibody - C-terminal (ab190078)
Anti-IL1 Receptor I antibody - C-terminal (ab190078) at 1/60 dilution + HL60 cell lysate at 35 µg