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Recombinant (Human) full length protein produced in E. coli
Our Abpromise guarantee covers the use of ab34843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.|
|IP||1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background.|
|ELISA||1/400 - 1/1200. (as detection antibody, using a monoclonal antibody for capture).|
|Neutralising||1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.|
|ICC/IF||Use at an assay dependent concentration.|