Overview

  • Product name
  • Description
    Rabbit polyclonal to IL10
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIL-10 (mouse Interleukin-10)

Properties

Applications

Our Abpromise guarantee covers the use of ab9735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (2.5 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required.

    ELISA:To detect mIL-10 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-10.

    Western Blot:To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
    • Tissue specificity
      Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
    • Sequence similarities
      Belongs to the IL-10 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    References for Anti-IL10 antibody (ab9735)

    This product has been referenced in:
    • Szelenyi ER & Urso ML Time-course analysis of injured skeletal muscle suggests a critical involvement of ERK1/2 signaling in the acute inflammatory response. Muscle Nerve 45:552-61 (2012). WB ; Mouse . Read more (PubMed: 22431089) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Spermophilus tridecemlineatus Tissue lysate - whole (Spleen)
    Loading amount
    50 µg
    Specification
    Spleen
    Gel Running Conditions
    Reduced Denaturing (12% Tris-Glycine)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Mr. James Bjork

    Verified customer

    Submitted Mar 01 2012

    Thank you for your interest in Abcam products.

    Are you interested in testing both ab66138 and ab9735 in squirrel? Do you know if the sequences are available for this species? We typically offer the testing discount if the homology with the i...

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    I am very pleased to hear you would like to accept our offer and test ab9735 in squirrel. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for squirrel and include this code in...

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    Thank you for your enquiry. The antibody originator has not tested this product in neutralization in vivo. All neutralization assays were performed in vitro. I am sorry I could not be more helpful. Please do not hesitate to contact us should yo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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