Overview

  • Product nameAnti-IL10 antibody
    See all IL10 primary antibodies
  • Description
    Rabbit polyclonal to IL10
  • Tested applicationsSuitable for: WB, ELISA, Neutralising, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 19/178 of Rat IL10 (available as ab9970.)

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Can be blocked with Recombinant rat IL10 protein (ab9970).
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of rIL-10 (30.0 ng/ml), a concentration of 0.45 - 0.8 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody images

  • ab9969 staining IL10 in murine immortalised bone marrow-derived macrophage by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.01% Triton X-100 and then blocked using 5% serum for 1 hour at 20°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 20°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/500 dilution.

    See Abreview

  • ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab9969 staining nucleolin in Raw 264.7 cells treated with spermine (ab120051), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermine, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120051) (spermine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References for Anti-IL10 antibody (ab9969)

This product has been referenced in:
  • Majkutewicz I  et al. Dimethyl fumarate attenuates intracerebroventricular streptozotocin-induced spatial memory impairment and hippocampal neurodegeneration in rats. Behav Brain Res 308:24-37 (2016). IF, IHC ; Rat . Read more (PubMed: 27083302) »
  • Cabrera-Pastor A  et al. In vivo administration of extracellular cGMP normalizes TNF-a and membrane expression of AMPA receptors in hippocampus and spatial reference memory but not IL-1ß, NMDA receptors in membrane and working memory in hyperammonemic rats. Brain Behav Immun 57:360-70 (2016). WB ; Rat . Read more (PubMed: 27189036) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Blocking step 1% Goat serum 1% BSA 5% NFDM in 1X PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 17 2014

Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nu...

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Thank you very much for contacting us with your enquiry.

The antibody ab9969 may be suitable for use with paraffin-embedded tissue, however we can't say for sure until this is tested. For cases like this, in which you're interested in trying ...

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Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Thank you for contacting us. As an isotype control for ab9969, I would recommend either ab37415 or ab27478. I hope this helps, please let me know if you have any additional questions.

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

Can you tell me more about your IL10-/- mice? You said you checked by ELISA. Did you use this same antibody?

Have you tried a...

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Thank you for contacting us and your interest in our products.


As discussed over the phone ab8918isamouse monoclonaland canbe used with an anti-mouse IgG secondary antibody.


ab39626 and ab9969 are bothrabbit polyclonals ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (immortalised bone marrow-derived macrophage)
Specification immortalised bone marrow-derived macrophage
Fixative Paraformaldehyde
Permeabilization Yes - .01% Triton X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. James Harris

Verified customer

Submitted Jan 31 2011

Thank you for your enquiry. The MW of rat IL10 is approximately 20 kDa. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"