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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Ion Exchange Chromatography
This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
BL: Use at an assay dependent dilution.
The antibody blocks IL10 induced proliferation on MC/9 cell line.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Belongs to the IL-10 family.
Information by UniProt
Cytokine synthesis inhibitory factor antibody
Anti-IL10 antibody [B-S10] images
ELISA - Anti-IL10 antibody [B-S10] - Azide free (ab47222) Image courtesy of an anonymous Abreview.
We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10.
ab47222 used at a 1/2000 dilution.
References for Anti-IL10 antibody [B-S10] (ab47222)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"