Anti-IL10 antibody [B-S10] (ab47222)

Overview

  • Product nameAnti-IL10 antibody [B-S10]
    See all IL10 primary antibodies
  • Description
    Mouse monoclonal [B-S10] to IL10
  • SpecificityThis antibody recognizes both natural and recombinant IL10.
  • Tested applicationsSuitable for: Blocking, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human IL10.

Properties

Applications

Our Abpromise guarantee covers the use of ab47222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking
ELISA
  • Application notesBL: Use at an assay dependent dilution.
    The antibody blocks IL10 induced proliferation on MC/9 cell line.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
    • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
    • Sequence similaritiesBelongs to the IL-10 family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    Anti-IL10 antibody [B-S10] images

    • We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10.
      ab47222 used at a 1/2000 dilution.

      See Abreview

    References for Anti-IL10 antibody [B-S10] (ab47222)

    ab47222 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Type Direct
    Sample Human Cell (Monocytes)
    Specification Monocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Feb 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"