Overview

  • Product name
    Anti-IL10 antibody [JES5-2A5]
    See all IL10 primary antibodies
  • Description
    Rat monoclonal [JES5-2A5] to IL10
  • Specificity
    ab189392 recognizes mouse IL10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse IL10.
    Database link: P18893

  • Positive control
    • Mouse small intestine and spleen tissues. Recombinant mouse IL10.

Properties

Applications

Our Abpromise guarantee covers the use of ab189392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

Use 10 µl of the suggested working dilution to label 1x106 cells in 100 µl. Membrane permeabilization is required for this application.





ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity
    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL10 using ab189392 at 10 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL10 using ab189392 at 10 µg/ml.

  • Anti-IL10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL10

    Secondary
    F(ab)2 rabbit anti-rat IgG:HRP
    Developed using the ECL technique

    Predicted band size : 21 kDa

References

This product has been referenced in:
  • Li C  et al. Protective role of adenovirus vector-mediated interleukin-10 gene therapy on endogenous islet ß-cells in recent-onset type 1 diabetes in NOD mice. Exp Ther Med 11:1625-1632 (2016). Read more (PubMed: 27168782) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Paw)
Antigen retrieval step
None
Permeabilization
No
Specification
Paw
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 05 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina and Colon)
Antigen retrieval step
None
Permeabilization
No
Specification
Retina and Colon
Blocking step
Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Gluteraldehyde
Username

Premier Laboratory

Verified customer

Submitted Apr 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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