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Recombinant full length protein (Mouse)
ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.
Our Abpromise guarantee covers the use of ab33471 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 17 kDa.
We recommend incubating the membrane overnight in primary antibody.
|Flow Cyt||Use at an assay dependent concentration.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
This image is courtesy of an anonymous abreview.
Blocked with 5% milk for 18 hours at 4°C.
Immunohistochemistry (Frozen sections) analysis of rat brain tissue sections labeling IL10 with ab33471 at 1/1000 dilution. The tissue was fixed with paraformaldehyde and permeabilized with 0.1 M PBS with 3% Triton X. The tissue was blocked with 10% serum for 1 hour at 24°C, followed by incubation with Anti-IL10 antibody [JES5-2A5] (ab33471) in 0.1M PBST with 10% donkey serum for 24 hours at 4°C. Alexa Fluor 488® secondary antibody was used at 1/1000 dilution. The IL-10 was visible in the M2 macrophages in the choroid plexus. Nucleus was stained with DAPI.