Overview

  • Product name
    Anti-IL10 antibody [JES5-2A5]
    See all IL10 primary antibodies
  • Description
    Rat monoclonal [JES5-2A5] to IL10
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Mouse)

  • Positive control
    • WB: LPS treated mouse liver lysate. LPS treated mouse hepatocyte lysate. IHC-Fr: Rat brain tissue.
  • General notes

    ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Purified from TCS.
  • Primary antibody notes
    ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.
  • Clonality
    Monoclonal
  • Clone number
    JES5-2A5
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa.

We recommend incubating the membrane overnight in primary antibody.

Flow Cyt Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity
    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody [JES5-2A5] images

  • All lanes : Anti-IL10 antibody [JES5-2A5] (ab33471) at 1/500 dilution

    Lane 1 : Mouse liver whole cell lysate (treated with LPS 5mg/kg)
    Lane 2 : Mouse hepatocytes (Treated with LPS 100 ng/ml)

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-rat (HRP) at 1/1000 dilution
    Developed using the ECL technique

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific binding).

    Exposure time : 45 seconds

    This image is courtesy of an anonymous abreview.

    Blocked with 5% milk for 18 hours at 4°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) analysis of rat brain tissue sections labeling IL10 with ab33471 at 1/1000 dilution. The tissue was fixed with paraformaldehyde and permeabilized with 0.1 M PBS with 3% Triton X. The tissue was blocked with 10% serum for 1 hour at 24°C, followed by incubation with Anti-IL10 antibody [JES5-2A5] (ab33471) in 0.1M PBST with 10% donkey serum for 24 hours at 4°C. Alexa Fluor 488® secondary antibody was used at 1/1000 dilution. The IL-10 was visible in the M2 macrophages in the choroid plexus.  Nucleus was stained with DAPI.

References for Anti-IL10 antibody [JES5-2A5] (ab33471)

This product has been referenced in:
  • Lund AW  et al. Lymphatic vessels regulate immune microenvironments in human and murine melanoma. J Clin Invest 126:3389-402 (2016). Read more (PubMed: 27525437) »
  • Chen L  et al. Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways. Sci China Life Sci 58:531-40 (2015). Read more (PubMed: 25951933) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse liver, mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
LPS 5mg/kg iv for liver, 100 ng/ml for hepatocytes
Specification
mouse liver, mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 25 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 13 2014

Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Thank you for contacting us.

We haven't tested this antibody in paraffin embedded tissue sections (IHC-P), only frozen sections from mouse. However, IL10 is produced and secreted by a variety of cell lines, including T-cells, macrophages, ma...

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We typically load 10 - 20 nanograms of purified recombinant protein in gels for western blots. I have contacted the laboratory to find out the exact amount that was used for the blot on the datasheet but I expect it will be within that range.

Thank you for contacting us.

We recommend ab18412.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply and for sending your protocol information and image. We do guarantee ab33471 to work in Western blot with mouse lysates, so we are happy to issue you a refund for this purchase. I have requested the refund to our accoun...

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According to our records, ab33471 was proving difficult to use in IHC and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order for us to resolve the problem. If the ...

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Thank you for contacting us, and I am sorry that this antibody has not produced expected results. We are happy to offer a refund if a product does not work in a tested species and application within 6 months of purchase. Do you have your order number? ...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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