Overview

  • Product nameIL10 Human ELISA Set (without plates)
    See all IL10 kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    400 pg/ml - 12.5 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 Human in vitro ELISA Set is designed for the quantitative measurement of IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human IL10 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 5 pg / ml

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL-10
    • IL10
    • IL10_HUMAN
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • IL10 Human ELISA Set (without plates) images

    • IL-10 Standard curve ranging from 12.5 to 400 pg/mL

    References for IL10 Human ELISA Set (without plates) (ab48485)

    ab48485 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab48485.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"