- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-IL10RA antibody
See all IL10RA primary antibodies -
DescriptionRabbit polyclonal to IL10RA
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human IL10RA.
(Peptide available as ab109885.)
-
Positive control
- This antibody gave a positive signal in HUVEC whole cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab94811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
-
FunctionReceptor for IL10; binds IL10 with a high affinity.
-
Tissue specificitySpleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.
-
Involvement in diseaseInflammatory bowel disease 28
-
Sequence similaritiesBelongs to the type II cytokine receptor family.
-
Cellular localizationMembrane.
- Information by UniProt
-
Database links
- Entrez Gene: 3587 Human
- Omim: 146933 Human
- SwissProt: Q13651 Human
- Unigene: 504035 Human
-
Alternative names
- CD210 antibody
- CDw210a antibody
- CDw210a antigen antibody
see all
Images
-
Western blot - Anti-IL10RA antibody (ab94811)Anti-IL10RA antibody (ab94811) at 1 µg/ml + HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-IL10RA antibody (ab94811)ICC/IF image of ab94811 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94811 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab94811 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"