Anti-IL10RA (phospho Y496) antibody (ab63406)

Overview

  • Product nameAnti-IL10RA (phospho Y496) antibody
    See all IL10RA primary antibodies
  • Description
    Rabbit polyclonal to IL10RA (phospho Y496)
  • Specificityab63406 detects endogenous levels of IL10RA only when phosphorylated at tyrosine 496.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human IL10RA around the phosphorylation site of tyrosine 496 (K-G-YP-L-K).

  • Positive control
    • HuvEc cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab63406 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • FunctionReceptor for IL10; binds IL10 with a high affinity.
  • Tissue specificitySpleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.
  • Involvement in diseaseInflammatory bowel disease 28
  • Sequence similaritiesBelongs to the type II cytokine receptor family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD210 antibody
    • CDw210a antibody
    • CDw210a antigen antibody
    • HIL 10R antibody
    • HIL10R antibody
    • I10R1_HUMAN antibody
    • IL 10R 1 antibody
    • IL 10R A antibody
    • IL 10R antibody
    • IL 10R1 antibody
    • IL-10 receptor subunit alpha antibody
    • IL-10R subunit 1 antibody
    • IL-10R subunit alpha antibody
    • IL-10R1 antibody
    • IL-10RA antibody
    • IL10R antibody
    • IL10R1 antibody
    • IL10RA antibody
    • Interleukin 10 receptor alpha antibody
    • Interleukin 10 receptor alpha chain antibody
    • Interleukin-10 receptor subunit 1 antibody
    • Interleukin-10 receptor subunit alpha antibody
    see all

Anti-IL10RA (phospho Y496) antibody images

  • All lanes : Anti-IL10RA (phospho Y496) antibody (ab63406) at 1/500 dilution

    Lane 1 : HuvEc cell extracts
    Lane 2 : HuvEc cell extracts with immunising peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-IL10RA (phospho Y496) antibody (ab63406)

ab63406 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"