Anti-IL-11 antibody (ab76589)
Key features and details
- Rabbit polyclonal to IL-11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-11 antibody
See all IL-11 primary antibodies -
Description
Rabbit polyclonal to IL-11 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human IL-11 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90835) -
Positive control
- This antibody gave a positive signal in Human Plasma Total Protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3589 Human
- Omim: 147681 Human
- SwissProt: P20809 Human
- Unigene: 467304 Human
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Alternative names
- Adipogenesis inhibitory factor antibody
- AGIF antibody
- IL 11 antibody
see all
Images
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Anti-IL-11 antibody (ab76589) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutesThe 27 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to IL-11.
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Anti-IL-11 antibody (ab76589) at 1 µg/ml + Recombinant human IL-11 protein (ab73620) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab76589 has been referenced in 4 publications.
- Tian Y et al. Immunosuppressants Tacrolimus and Sirolimus revert the cardiac antifibrotic properties of p38-MAPK inhibition in 3D-multicellular human iPSC-heart organoids. Front Cell Dev Biol 10:1001453 (2022). PubMed: 36438566
- Wang Y et al. Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer. J Cell Biochem 119:1841-1854 (2018). PubMed: 28815726
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Jiang Y et al. Downregulation of SPARC expression inhibits the invasion of human trophoblast cells in vitro. PLoS One 8:e69079 (2013). WB . PubMed: 23935929