Overview

  • Product nameAnti-IL12 antibodySee all IL12 primary antibodies ...
  • Description
    Goat polyclonal to IL12
  • SpecificityThis anti-IL-12 will recognize both the p35 and p40 monomer subunits and the p70 heterodimer.
  • Tested applicationsIHC-P, Flow Cyt, WB, ELISA, Neutralising more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-12 (human Interleukin-12)

Properties

Applications

Our Abpromise guarantee covers the use of ab9992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent dilution.
WB Use at an assay dependent concentration. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required.

Target

  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P29459 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CLMF antibody
    • CLMF p35 antibody
    • CLMF p40 antibody
    • CLMF1 antibody
    • CLMF2 antibody
    • CTL maturation factor (TcMF) antibody
    • Cytotoxic lymphocyte maturation factor 1 antibody
    • Cytotoxic lymphocyte maturation factor 1, p35 antibody
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
    • IL 2 subunit p40 antibody
    • IL-12 subunit p35 antibody
    • IL-12A antibody
    • IL-12B antibody
    • IL12, subunit p40 antibody
    • IL12A antibody
    • IL12A_HUMAN antibody
    • IL12B antibody
    • IL35 subunit antibody
    • Interleukin 12 alpha chain antibody
    • Interleukin 12 beta chain antibody
    • Interleukin 12 subunit beta antibody
    • Interleukin 12, p40 antibody
    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
    • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody
    • Interleukin 12B antibody
    • Interleukin-12 subunit alpha antibody
    • Natural killer cell stimulatory factor 1 antibody
    • Natural killer cell stimulatory factor 1, 35 kD subunit antibody
    • natural killer cell stimulatory factor 2 antibody
    • Natural killer cell stimulatory factor, 40 kD subunit antibody
    • NF cell stimulatory factor chain 1 antibody
    • NFSK antibody
    • NK cell stimulatory factor chain 1 antibody
    • NK cell stimulatory factor chain 2 antibody
    • NKSF antibody
    • NKSF1 antibody
    • NKSF2 antibody
    • P35 antibody
    • T cell stimulating factor (TSF) antibody
    see all

Anti-IL12 antibody images

  • IHC image of ab9992 staining in human astrocytoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9992, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IL12 antibody (ab9992)

This product has been referenced in:
  • Al-Sammak F  et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . Read more (PubMed: 24069393) »
  • Schlaepfer E & Speck RF Anti-HIV activity mediated by natural killer and CD8+ cells after toll-like receptor 7/8 triggering. PLoS ONE 3:e1999 (2008). Neutralising ; Human . Read more (PubMed: 18431484) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (human peripheral blood mononuclear cells)
Loading amount 100 µg
Specification human peripheral blood mononuclear cells
Treatment 5hr PMA (50 ng/mL) + Ionomycin (1 ug/mL) + monensin 2uM
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

Submitted Jun 06 2011

I am please to confirm that the products ab9992 and ab12645 will recognise both the p35 and p40 monomer subunits and the p70 heterodimer in western blotting. I hope this information will be useful to the customer.

Unfortunately, the cross-reactivity of this antibody with ovine IL-12 has not been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"