Anti-IL12 p35 + IL12 p70 antibody [B-T21] (FITC) (ab27401)

Overview

  • Product name
    Anti-IL12 p35 + IL12 p70 antibody [B-T21] (FITC)
  • Description
    Mouse monoclonal [B-T21] to IL12 p35 + IL12 p70 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab27401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Interleukin 12 (IL12) is an important cytokine produced by a variety of immune effector cells that leads to a type 1 helper T cell (Th1) response. IL12 also directs T cells to the skin via induction of cutaneous lymphocyte antigen (CLA) expression. Interleukin 12 is a heterodimeric cytokine (p70), composed of two covalently linked subunits, p35 and p40.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • CLMF1 antibody
    • CLMF2 antibody
    • IL 12 subunit p35 antibody
    • IL 12 subunit p40 antibody
    • IL12A antibody
    • IL12B antibody
    • Interleukin 12A antibody
    • Interleukin 12B antibody
    see all

Images

  • Overlay histogram showing peripheral blood lymphocytes cells stained with ab27401 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27401, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (0.5µg/1x106 cells ). Acquisition of >5,000 events was performed.

References

ab27401 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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