Overview

  • Product nameAnti-IL12 p40 antibody [1-1A4]
    See all IL12 p40 primary antibodies
  • Description
    Mouse monoclonal [1-1A4] to IL12 p40
  • SpecificityRecognises p35/40 heterodimer and p40. Used against CCHO and Baculovirus derived recombinant IL-12. Reacts with stimulated and unstimulated monocytes.
  • Tested applicationsSuitable for: ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human interleukin-12 produced in Baculovirus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPhosphate buffered saline
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number1-1A4
  • MyelomaSp2
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

Target

  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Involvement in diseaseDefects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLMF antibody
    • CLMF p40 antibody
    • CLMF2 antibody
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
    • IL 12 subunit p40 antibody
    • IL 12B antibody
    • IL-12 subunit p40 antibody
    • IL-12B antibody
    • IL12 subunit p40 antibody
    • IL12B antibody
    • IL12B_HUMAN antibody
    • interleukin 12 beta chain antibody
    • Interleukin 12 p40 antibody
    • Interleukin 12 subunit beta antibody
    • Interleukin 12B antibody
    • Interleukin-12 subunit beta antibody
    • natural killer cell stimulatory factor 40 kD subunit antibody
    • NK cell stimulatory factor chain 2 antibody
    • NKSF antibody
    • NKSF2 antibody
    • p40 antibody
    see all

References for Anti-IL12 p40 antibody [1-1A4] (ab50)

This product has been referenced in:
  • Carter RW  et al. Production and characterization of monoclonal antibodies to human interleukin-12. Hybridoma 16:363-9 (1997). Read more (PubMed: 9309427) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. For both ELISA, I would recommend starting at a dilution of 1:500 - 1:1000 and optimizing based upon results. For IHC, I would recommend using the dilution range of 1:20 - 1:200 and optimizing based upon results. If you have...

Read More

The source of this antibody is away on vacation unitl mid November, so I am unable to obtain the details you require. In the meantime, one of her colleagues has suggested the following reference: Carter, R.W. et al. Hybridoma 16 (4) p363-369 (1997).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"