Anti-IL12 p40 antibody (ab62822)

Overview

• Product name
  Anti-IL12 p40 antibody
  See all IL12 p40 primary antibodies
• Description
  Goat polyclonal to IL12 p40
• Tested applications
  Suitable for: IHC-P, WB, ELISAmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Cynomolgus monkey, Rhesus monkey
• Immunogen

  Synthetic peptide:

  C-QGKSKREKKDRVFTD

  , corresponding to internal sequence amino acids 278-292 of Human IL12 p40

  Run BLAST with Run BLAST with
• Positive control
  • Human Liver lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  ab62822 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
• Positive Controls
  • Recombinant Human IL12 p80 protein (ab63226)
  • Recombinant Human IL12 protein (ab68134)
  • Recombinant human IL12 protein (ab89013)

Applications

Target

• Function
  Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
• Involvement in disease
  Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
• Sequence similarities
  Belongs to the type I cytokine receptor family. Type 3 subfamily.
  Contains 1 fibronectin type-III domain.
  Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
• Post-translational
  modifications
  Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
• Cellular localization
  Secreted.

• Target information above from: UniProt accession P29460 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 3593 Human
  • Omim: 161561 Human
  • SwissProt: P29460 Human
  • Unigene: 674 Human
• Alternative names
  • CLMF antibody
  • CLMF p40 antibody
  • CLMF2 antibody

  • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
  • IL 12 subunit p40 antibody
  • IL 12B antibody
  • IL-12 subunit p40 antibody
  • IL-12B antibody
  • IL12 subunit p40 antibody
  • IL12B antibody
  • IL12B_HUMAN antibody
  • interleukin 12 beta chain antibody
  • Interleukin 12 p40 antibody
  • Interleukin 12 subunit beta antibody
  • Interleukin 12B antibody
  • Interleukin-12 subunit beta antibody
  • natural killer cell stimulatory factor 40 kD subunit antibody
  • NK cell stimulatory factor chain 2 antibody
  • NKSF antibody
  • NKSF2 antibody
  • p40 antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols