The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceIL12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL12 is a potent stimulator of NK cells. IL12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses.
Overlay histogram showing peripheral blood lymphocytes cells stained with ab27376 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27376, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (0.5µg/1x106 cells ). Acquisition of >5,000 events was performed.