The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Suitable for intra cytoplasmic flow cytometry.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceIL12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL12 is a potent stimulator of NK cells. IL12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses.
References for Anti-IL12 p40 + IL12 p70 antibody [B-P40] (ab27379)
This product has been referenced in:
Verreck FA et al. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria. Proc Natl Acad Sci U S A101:4560-5 (2004).
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