The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesNeut: Use 8000 neutralizing units/mg protein. One neutralizing unit is defined as the amount of IgG sufficient for a 50% inhibition of IFN gamma synthesis by human PBMC (2 x 105 cells) stimulated with 1 ng Human IL12.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 and 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceIL12 p70 is a disulfide bonded heterodimer of the IL12 40 kDa and 35 kDa subunits.